Custom Matched Monoclonal Antibody Service丨Zoonbio

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antigen preparation	animal immunity	cell fusion	cell supernatant pairing	antibody purification	antibody matching Detection
immunogenic analysis antigen design peptide synthesis or protein preparation	3-4 times of animal immunization serum titer higher than 100,000	preparation of animal spleen cells cell fusion fusion cell screening	matching of supernatants of multiple monoclonal hybridoma cells	BMP produces antibody Protein A/G purification	ELISA detection of ligand sensitivity
Delivery criteria 1.Residual immunogen protein. 2.2-5 positive clonal cell lines (containing 1-2 pairs of ligand positive clonal cell lines), 2 frozen tubes per cell. 3.At least one pair of ligands, 1-3 mg for each antibody. Supplementary Note By entrusting Zhongding Biology to prepare immunogen and screening positive cell lines, we can provide cell culture supernatant for customers to verify the specificity and affinity index of cell lines.

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Case Background

According to the sequence provided by the customer, the antigen protein X was recombined and expressed for the customer; the mice were immunized with protein X as antigen, and the ligand cell lines A and B were screened by matching the supernatant of fusion cell culture, then the antibodies A and B were purified, and the pairing sensitivity of A and B was detected by sandwich ELISA.



detection of purity of antibodies A and B by SDS-PAGE M: standard molecular weight of protein Lane 1:antibody A Lane 2:antibody B

Empirical Conclusion

The sensitivity of pairing detection of purified antibody A with labelled antibody B and purified antibody B with labelled antibody A can reach 0.97pg/ml.

Custom Matched Monoclonal Antibody Service

Service Flow

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