Zoonbio's chicken yolk antibody preparation technology service can provide a variety of forms of services for scientific research users.
It can provide chicken anti-serum service as well as delivery of egg yolk antibody.
It can also purify egg yolk and serum according to customer requirements.
The preparation of egg yolk antibody can deliver 5-10 eggs, the titer of egg yolk antibody is more than 1:20,000, and the titer of chicken serum is more than 1:100,000.
Zoonbio has experienced immunological technicians, standardized operation process, can provide users with low-cost, high-quality antibody products and technical services.
At the same time, Zoonbio is good at the preparation of antibodies against small molecule antigens, and has unique core technology for the coupling of pesticides, small molecule compounds, heavy metal antigens and immunoassay.
Immunization of two Roman chickens during laying period requires antigen concentration not less than 0.5 mg/ml, purity more than 85%, and protein content not less than 3 mg if customers provide antigen. Serum titers were tested 7 days after immunization and eggs were collected. Normally, 15 to 20 eggs were collected for reserve.
Purification of Yolk Antibody
A.yolk purification
(1)ammonium sulfate precipitation method
(2)antigen affinity purification
B.serum purification
(1)ammonium sulfate precipitation method
(2)antigen affinity purification
Related services:
determination of antibody affinity service
preparation and coupling of small molecular antigens
humanization of antibodies service