GENERAL INFORMATION
Gene Name Synonym: coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCPCoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBD
Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S2 ECD) (YP_009724390.1) (Ser686-Pro1213) was expressed with a polyhistidine tag at the C-terminus.
Source: 2019-nCoV
Expression Host: Baculovirus-Insect Cells
QC Testing
Purity: >90 % as determined by SDS-PAGE
Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
Predicted N terminal: Ser 686
Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S2 ECD, His tag) consists of 539 amino acids and predicts a molecular mass of 59.37 kDa.
Formulation: Lyophilized from sterile 20 mM PB, 300 mM NaCl, 10 % glycerol, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
Usage Guide
Stability & Storage: Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Avoid repeated freeze-thaw cycles.
Reconstitution: Detailed reconstitution instructions are sent along with the products.


SDS-PAGE:

protein case




Related reading:    COVID-19 Related Products Collection