| GENERAL INFORMATION | |
| Gene Name Synonym： | coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBD |
| Protein Construction: | A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit) (YP_009724390.1) (Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus. |
| Source: | 2019-nCoV |
| Expression Host: | Baculovirus-Insect Cells |
| QC Testing | |
| Purity: | ＞90 % as determined by SDS-PAGE |
| Bio Activity： | Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 10μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (S1, His tag), the EC50 of 2019-nCoV Spike Protein (S1, His tag) is 300-600 ng/mL.
|
| Endotoxin: | ＜ 1.0 EU per μg protein as determined by the LAL method. |
| Predicted N terminal: | Val 16 |
| Molecular Mass: | The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His tag) consists of 681 amino acids and predicts a molecular mass of 76.45 kDa. |
| Formulation: | Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements. |
| Usage Guide | |
| Stability & Storage: | Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. |
| Avoid repeated freeze-thaw cycles. | |
| Reconstitution: | Detailed reconstitution instructions are sent along with the products. |
SDS-PAGE:
