According to the RdRp, e and N genes published by who for covid-19 detection, Zoonbio synthesized corresponding probes, primers and positive control plasmids. The use of the internal reference gene can increase the validity of the samples.
| Primer Probe for COVID-19 Nucleic Acids | ||||||
| Gene Name | Oligo Name | Oligo Sequence(5'-3') | Length (nt) | 5'mod | 3'mod | Price |
| RdRP gene | RdRP_SARSr-F2 | GTGARATGGTCATGTGTGGCGG | 22 | Consultation | ||
| RdRP_SARSr-R1 | CARATGTTAAASACACTATTAGCATA | 26 | ||||
| RdRP_SARSr-P2 | CAGGTGGAACCTCATCAGGAGATGC | 25 | FAM | BHQ-1 | ||
| E gene | E_Sarbeco_F1 | ACAGGTACGTTAATAGTTAATAGCGT | 26 | |||
| E_Sarbeco_R2 | ATATTGCAGCAGTACGCACACA | 22 | ||||
| E_Sarbeco_P1 | ACACTAGCCATCCTTACTGCGCTTCG | 26 | FAM | BHQ-1 | ||
| N gene | N_Sarbeco_F1 | CACATTGGCACCCGCAATC | 19 | |||
| N_Sarbeco_R1 | GAGGAACGAGAAGAGGCTTG | 20 | ||||
| N_Sarbeco_P1 | ACTTCCTCAAGGAACAACATTGCCA | 25 | CY5 | BHQ-3 | ||
| Human tissue reference gene | GAPDHF | TCAAGAAGGTGGTGAAGCAGG | 21 | |||
| GAPDHR | CAGCGTCAAAGGTGGAGGAGT | 21 | ||||
| GAPDHP | CCTCAAGGGCATCCTGGGCTACACT | 25 | ROX | BHQ-2 | ||
| Positive control plasmid | The partial sequences of four genes, RdRp, e, N containing covid-19 and GAPDH in human, can be used as RT-PCR positive control. | |||||
WHO recommended links:Diagnostic detection of Wuhan coronavirus 2019 by real-time RT-PCR – Charité, Berlin Germany (17 January 2020)
Related reading： COVID-19 Related Products Collection