CRISPR/Cas9 is an important tool for targeting gene-specific DNA modification in recent years. It has been used more and more widely. Technology has developed into a popular tool for genome editing. Compared with TALEN and ZNF technologies, CRISPR/Cas9 system is more convenient to construct and use vectors. The specificity of gene knockout is determined by gRNA rather than protein, thus avoiding the complex process of plasmid assembly and construction, and even making it possible to knock out multiple genes simultaneously. Moreover, experiments show that the gene knockout efficiency of CRISPR/Cas9 system is equivalent to or better than that of TALEN and ZNF. At present, the specificity and other characteristics of Cas9 need to be further studied, but due to its extremely simple operation, the application of this technology will greatly simplify breeding, genetic mutation repair, and the difficulty of various gene research. It can complete RNA-oriented DNA recognition and editing, and provides a new platform for more efficient gene editing technology.
| service content | Service price | Service cycle |
|---|---|---|
| construction of CRISPR/Cas9 vector(conventional system) | inquiry | 3-4 weeks |
| construction of CRISPR/Cas9 vector(double incision system) | inquiry | 3-4 weeks |
| CRISPR/Cas9 Co-rotating system(integration of sgRNA and Cas9) | inquiry | 4-6 weeks |
| construction of adenovirus vector CRISPR/Cas9 | inquiry | 4-6 weeks |
| detection of CRISPR/Cas9 vector activity | inquiry | 4-6 weeks |
Related services：
Site-Directed Mutagenesis Service
TA Cloning And Sequencing Service
Gene Synthesis Services