After genomic DNA and total RNA were extracted from cells, proteins, polysaccharides and nucleic acids of various sizes were still mixed.
The process of removing these "impurities" is the process of nucleic acid purification.
In order to prevent denaturation and degradation of nucleic acid macromolecule, it is necessary to operate at low temperature of 0-4 C in the separation and purification of nucleic acid.
Hydrolysis of nuclease is a serious obstacle to the preparation of active nucleic acid macromolecules in the past.
Nowadays, decontamination agents or EDTA, 8-hydroxyquinoline and sodium citrate are commonly used to remove activator Mg2+, which can inhibit the activity of nuclease and ensure the integrity of nucleic acid macromolecules in the purification process.
Zoonbio can extract genomic DNA and total RNA from a variety of materials. Because the amount of genomic DNA and total RNA extracted from different materials is different, even from the same material with different freshness, the difference is quite large.
Therefore, when providing materials, it is necessary to ensure that the experimental materials are fresh and sufficient, and to use specific methods for preservation and transportation.
| Extraction and Purification of Genomic DNA | ||||
| Service Items | Material Rquirements | Getting DNA Quantity | cycle | offer |
| extraction of bacterial genomic DNA | 1-2ml fresh bacterial liquid in logarithmic growth period | 1-2ug | 5 days | inquiry |
| extraction of cell genomic DNA | 1 x 106 fresh or frozen cells | ≈10ug | 5 days | |
| extraction of blood genomic DNA | 1 ml fresh or liquid nitrogen frozen sample | ≈10ug | 5 days | |
| extraction of tissue genomic DNA | 100 mg fresh or - 20 degree frozen samples | ≈10ug | 5 days | |
| We use imported high-quality kits to extract genomic DNA, which can ensure that the genomic DNA obtained can meet the requirements of high-standard experiments such as southern blot. If you have a large number of samples, please contact technical support staff and enjoy discounts! | ||||
| Extraction and Purification of Total RNA | ||||
| Service Items | Material Rquirements | Getting DNA Quantity | cycle | offer |
| total RNA extraction from bacteria | 1-2ml fresh bacterial liquid in logarithmic growth period | ≈10ug | 5 days | inquiry |
| total RNA extraction from cell | 1 x 106 suspension cultured or freshly collected cells | ≈1ug | 8 days | |
| total RNA extraction from blood | 1 ml fresh or liquid nitrogen frozen sample | ≈1ug | 8 days | |
| total RNA extraction from animal tissues | 100 mg fresh or liquid nitrogen frozen samples | ≈100ug | 8 days | |
| total RNA extraction from plant tissues | 100 mg fresh or liquid nitrogen frozen samples | ≈10ug | 8 days | |
| We use imported high-quality kits, clean space operation, the total RNA extracted can meet the requirements of Southern blot and qPCR and other high-standard experiments. If you have a large number of samples, please contact technical support staff to enjoy discounts! | ||||
Provide genomic DNA or total RNA samples, total concentration, total content, electrophoretic photographs, OD value determination results, etc.
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RT-PCR Service
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