Summary
Bimolecular Fluorescence Complementation(BiFC) is a new technology that can directly and rapidly determine the location and interaction of target proteins in living cells. By inserting exogenous fragments into specific sites of fluorescent proteins without affecting the fluorescence activity of GFP, the technique divides them into two non-fluorescent protein fragments, namely N-fragment and C-fragment. When the two proteins interacting with each other connect with N-fragment and C-fragment respectively, they form fusion proteins and express them together in living cells. The N-terminal and C-terminal fragments of photoproteins can be close to each other and form active fluorescent groups to emit fluorescence.
