Bimolecular Fluorescence Complementation(BiFC) is a new technology that can directly and rapidly determine the location and interaction of target proteins in living cells. By inserting exogenous fragments into specific sites of fluorescent proteins without affecting the fluorescence activity of GFP, the technique divides them into two non-fluorescent protein fragments, namely N-fragment and C-fragment. When the two proteins interacting with each other connect with N-fragment and C-fragment respectively, they form fusion proteins and express them together in living cells. The N-terminal and C-terminal fragments of photoproteins can be close to each other and form active fluorescent groups to emit fluorescence.


BiFC System Selection

Split fluorescent protein(YFP) common type:The system is suitable for the study of protein interaction with strong protein interaction. The fluorescence intensity of YFP is controlled at a low level and is not disturbed by background factors.
Split fluorescent protein(YFP) enhanced type:The system is suitable for the study of protein interaction, such as weak interaction between membrane proteins. YFPn and YFPc are edited manually, and YFP fluorescence is enhanced. It is convenient to observe weak interaction fluorescence signals. At the same time, 206 amino acid A was mutated into K, which reduced the interference of background and other factors, and the results were true and reliable.
The Split luciferase (LUC) system:The system can observe and photograph the living system, especially for quantitative analysis. It is suitable for the study of interaction between proteins affected by third-party substances. For example, the strength of protein interaction can be regulated by hormones and small molecular compounds, and the changes of interaction can be determined by the observation of exogenous hormone compounds.

Service Items

service content explain cycle delivery of products
construction of expression plasmid gene optimization
subclonal design
synthetic gene
subcloning to vectors
vector construction
1-2 weeks
expression plasmid
gene synthesis report
agrobacterium tumefaciens
BiFC experiment plasmid transformation of agrobacterium tumefaciens
agrobacterium tumefaciens transfected tobacco
transient expression of interacting proteins
observation and photography of experimental results
expression identification
3-4 weeks
BiFC experimental report
the original picture of the experiment



control result corresponding to B and A1/A2/A3 tobacco BiFC


experimental results of B and A1/A2/A3 tobacco BiFC

Fig. 1 shows that there is no fluorescence signal in the control group. Fig. 2 shows that B-YC and A2-YN and YN-A3 are co-transfected instantaneously, and obvious fluorescence can be observed. It can be concluded that B protein interacts with A2 and A3 proteins. After co-transfection of B-YC and A1-YN, weak fluorescence can be observed, which indicates that B and A1 also interact.

Related services:

Co-IP service

GST Pull-Down service

physicochemical analysis of proteins service