co-immunoprecipitation(Co-IP) service丨Zoonbio

co-immunoprecipitation(Co-IP) service

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Summary

Co-immunoprecipitation (Co-IP) is a classical method for studying protein interaction, which is based on the specificity between antibodies and antigens. Co-IP detects the binding of two proteins, which conforms to the actual situation in vivo and has high reliability. It is often used to verify the interaction between two known proteins and to find unknown proteins interacting with known proteins.

experimental process

High success rate

Using imported reagent kit, Protein A magnetic beads have high adsorption efficiency, and the success rate of the experiment is more than 90%.

High sensitivity

Using silver dyeing technology, the strips are clearer and the experimental results are more reliable.

High accuracy rate

Mature experimental mechanism, scientific control group settings, to maximize the accuracy of the experimental results.

One-stop service

Three-dimensional upstream and downstream service system, perfect gene, protein and antibody service platform, real one-stop service experience.

Service Items

package type	service content	starting material	deliver products	cycle
regular package	Co-IP experiment mass spectrometry detection	protein sequence detection antibody	Co-IP experiment report protein experiment report	2-3 weeks
overexpression of Co-IP in cells	gene synthesis cell transfection, overexpression of target protein Co-IP experiment mass spectrometry detection	protein sample detection antibody	gene synthesis report protein expression report Co-IP experiment report mass spectrometry detection report	5-6 weeks
Co-IP of tobacco plants	Gene synthesis Transient expression in tobacco Co-IP experiment Mass spectrometry	protein sample detection antibody	gene synthesis report Co-IP experiment report mass spectrometry detection report	5-6 weeks

case

experimental purpose:A-flag and B-GFP genes were co-transfected into tobacco plants, and the interaction of A and B proteins was verified by Co-IP experiment.
experiment setup:A-flag and B-GFP genes were co-transfected into tobacco plants as experimental group, and A-flag and GFP genes were co-transfected into tobacco plants as control group.
experimental result:


Co-IP results showed that A-flag and B-GFP genes were expressed after co-transfection of tobacco plants; A-flag and GFP genes were also expressed after co-transfection of tobacco plants; A protein did not bind to GFP protein after co-transfection of A-flag and GFP genes; B protein interacted with A protein after co-transfection of A-flag and B-GFP genes, and was precipitated by Anti-Flag and detected by Anti-GFP.
empirical conclusion: In this experiment, anti-Flag was used to carry out IP experiment, and anti-GFP was used to verify WB. The interaction between B protein and A protein was successfully detected.