DNA Pull Down is the main tool for studying the interaction between DNA and protein in vitro. DNA was labeled with biotin. DNA was fixed to magnetic beads by non-covalent binding of biotin and Streptomyces affinity. When protein and DNA were combined, DNA-protein complexes were formed. The complexes were eluted from magnetic beads. The DNA-binding proteins were detected by Western Blot validation and mass spectrometry.

experimental principle

experimental principle  

Service Items

service items service content initial material delivery of materials
DNA Pull-Down Probe preparation
nucleoprotein extraction
DNA Pull-Down
Western Blot
gene information (species, sequence or ID)
cell sample
protein information to be tested
detection of antibodies
experimental report of WB test results
RNA Pull-Down MS Probe preparation
nucleoprotein extraction
mass spectrometry detection
gene information (species, sequence or ID)
cell sample
experimental Report on Mass Spectrometric Detection Results

service flow

2.nucleoprotein extraction by probe preparation
3.Pull Down experiment
4.Western Blot or mass spectrometry identification of products of products
6.after-sale service

Related services:

yeast two-Hybrid technical service

ChIP assay service

RNA Pull-Down service