experimental principle
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DNA Pull Down is the main tool for studying the interaction between DNA and protein in vitro. DNA was labeled with biotin. DNA was fixed to magnetic beads by non-covalent binding of biotin and Streptomyces affinity. When protein and DNA were combined, DNA-protein complexes were formed. The complexes were eluted from magnetic beads. The DNA-binding proteins were detected by Western Blot validation and mass spectrometry.
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| service items | service content | initial material | delivery of materials |
| DNA Pull-Down | Probe preparation nucleoprotein extraction DNA Pull-Down Western Blot |
gene information (species, sequence or ID) cell sample protein information to be tested detection of antibodies |
experimental report of WB test results |
| RNA Pull-Down MS | Probe preparation nucleoprotein extraction SDS-PAGE mass spectrometry detection |
gene information (species, sequence or ID) cell sample |
experimental Report on Mass Spectrometric Detection Results |
| 1.sampling 2.nucleoprotein extraction by probe preparation 3.Pull Down experiment 4.Western Blot or mass spectrometry identification 5.delivery of products 5.delivery of products 6.after-sale service |
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