GST Pull-Down service丨Zoonbio

GST Pull-Down service

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Summary

Zoonbio's protein Expression Platform can help you get enough soluble protein. We use the imported GST Pull-Down kit to complete the protein interaction experiment quickly and accurately, and finally deliver the cell lysis supernatant and the original image of the experiment. You can complete the whole experiment by providing only the protein sequence.

experimental principle

Service Items

package type	service content	starting material	deliver products	cycle
routine set meal	GST Pull-Down experiment mass spectrometry detection	protein sample detection antibody	GST Pull-Down experiment report mass spectrometry detection report	1-2 weeks
prokaryotic expression GST-Pull-Down package	gene synthesis prokaryotic protein expression Pull-Down experiment mass spectrometry detection	protein sequence detection antibody	gene synthesis report protein expression report Pull-Down experiment report mass spectrometry detection report	5-6 weeks
eukaryotic expression GST-Pull-Down package	gene synthesis mammalian protein expression Pull-Down experiment mass spectrometry detection	protein sequence detection antibody	gene synthesis report protein expression report Pull-Down experiment report mass spectrometry detection report	7-8 weeks

case


SDS-PAGE analysis chart
M:protein molecular mass standard Lane M: Protein Marker Lane 1: column + GST-A protein elution Lane 2: column + B protein elution Lane 3: column + GST protein + B protein elution Lane 4: column + GST - A protein + B protein elution

WesternBlot analysis chart（Anti-GST）
M:protein molecular mass standard Lane M: Protein Marker Lane 1: column + GST-A protein elution Lane 2: column + B protein elution Lane 3: column + GST protein + B protein elution Lane 4: column + GST - A protein + B protein elution


WesternBlot analysis chart（Anti-His）
M:protein molecular mass standard Lane M: Protein Marker Lane 1: column + GST-A protein elution Lane 2: column + B protein elution Lane 3: column + GST protein + B protein elution Lane 4: column + GST - A protein + B protein elution

In order to verify the interaction between protein A and protein B, a multi-group control was set up to eliminate possible interference and ensure the correctness of the results. In Fig. 1, 2 and 3, it is known from Lane 1 that GST-A protein can bind to and be eluted from the curing column; from Lane 2, protein B does not bind to the curing column; from Lane 3, protein B does not bind to GST protein; and from Lane 4, protein B can bind to GST-A protein. Finally, it is concluded that protein A can interact with protein B.