Aantibiotic resistance genes(ARGs) is a class of genes that can make bacteria have antibiotic resistance. They are widely distributed in soil, sewage and other environments, and can migrate among bacterial flora through gene horizontal transfer.
ARGs is persistent and harmful in the environment, which has become an important issue in the new type of environmental pollution.
Zoonbio uses high-throughput fluorescence quantitative PCR technology to detect the expression profile of resistance genes in the environment and analyze the level of resistance genes in the environment.
1.extract DNA
2.testing DNA
3.qPCR
4.data analysis
sample requirements
1.Soil samples ≥ 200 mg, fecal samples ≥ 100 mg and sludge/sediment samples 5-10 g.
(1)Fresh samples are transported at room temperature.
(2)Freeze samples, dry ice transport, avoid repeated freezing and thawing.
2.Microbial DNA samples ≥ 2ug, concentration ≥ 300 ng/ul were transported at 4 ℃.
Powerful online and offline customer service team, one-to-one personalized docking, to achieve private customization experience.
Professional experimental team, super problem solving ability and data analysis ability, real-time customer problem solving.
The experiment is operated strictly according to SOP to ensure the quality is controllable.
High-throughput real-time fluorescence quantitative PCR system was used, and up to 380 resistance genes could be detected simultaneously, and their expression in one sample could be detected.
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