Species identification (strain identification) of microorganisms by DNA sequencing is a more advanced method than traditional biochemical identification.
DNA sequencing does not depend on the characteristics of strains, and can be used for all strains. It is faster and more accurate than traditional biochemical identification.
Zoonbio amplifies the highly variable region of ribosomal RNA (16S/26S/ITS sequence) by PCR, compares it with GeneBank or Eztaxon after sequencing, and judges whether it is a homologous strain by sequence similarity. Generally, the homologous strain can be considered as a homologous strain if the sequence similarity is more than 97%. Zoonbio primers optimized by a large number of experiments are suitable for more than 99% of microorganisms, and the identification success rate is more than 95%.
|service items||service description||service cycle|
|16S partial sequence identification of rDNA||In the light of 6S Partial Sequence Identification of rRNA(500bp)||8 Working day|
|identification of rDNA complete sequence||In the light of 6S full-length sequencing of rRNA(1500bp)||10 Working day|
|identification of fungi 26S rDNA||sequencing of 26S rDNA D1/D2 fragment(500bp)||10 Working day|
|identification of fungi ITS||ITS segment(300-1000bp)||10 Working day|
1.Sample form: fresh bacterial solution, inclined medium, flat medium or extracted DNA
(DNA concentration > 20ng/ul, volume > 20ul).
2.The identification results can generally identify species.Individual strains can only be
identified as genus because of the lack of data related to microbial database.
3.Because the impurity of the strain will lead to a set of peaks in sequencing, the
identification results will be erroneous.
4.For some pathogenic bacteria, be sure to specify in advance.
Experiments report: Including photos of PCR products, results of NCBI evolutionary tree alignment
for strain identification, sequencing color peak maps and sequence base reports.
Total DNA was extracted from animal tissues, blood, plant stems, leaves and flowers.
Specific primers were designed to amplify mitochondrial cytochrome oxidase subunit I (COI).
About 700 BP of PCR products were obtained.
After sequencing, data were compared and analyzed in NCBI or corresponding animal and plant databases to determine the genus to be detected.
According to different samples, specific sample size and delivery requirements, please consult company customer service personnel.
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