Summary

The resequencing project builds a database and sequences the genomic DNA of the target sample, and then compares the sequencing data to the reference genome through bioinformatics analysis, so as to obtain a large amount of genetic variation information. Common application directions are GWAS, mutation micropoint mapping, genetic evolution and so on.

Compared with marker detection methods such as chip KASP, re-sequencing items have two main differences.
Resequencing can detect all the genetic variations detected in target samples, many of which may be new mutation sites, but chip and KASP technology can only detect mutation sites that have been designed and verified.

Resequencing can discover a large number of genetic mutation sites. Generally speaking, the chip can achieve tens of thousands to hundreds of thousands of marker densities, but re-sequencing can generally produce more than one million marker densities.

Therefore, re-sequencing is a good choice for items with high marker density or special population polymorphism.

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