Summary

Zoonbio based on industry standards and real-time fluorescence quantitative pcr, provides screening and strain identification services for plants and their processed products for universities, scientific research institutions and enterprise customers.

At present, there are soybean, maize, rapeseed, rice, cotton, potato, flax, beet, alfalfa, tomato, papaya, apple, chicory, glume cutting, tobacco, plum, melon, wheat, eggplant and Eucalyptus that can provide screening and detection services for genetically modified ingredients.

At present, soybean, maize, rape, cotton, rice, potato, flax and sugar beet can provide identification services for genetically modified strains.

Screening and Detection of Transgenic Components

After extracting the sample DNA, real-time fluorescence PCR was used to amplify the specific fragments of the selected genes or strains. According to the real-time fluorescence amplification curve, the samples were judged whether or not there were genetically modified components.

species selected genes price
soybean and its processed products endogenous gene,pCaMV35S,pFMV35S,tNOS, BAR, PAT,GOX,CP4-EPSPS,CTP2-CP4-EPSPS,tE9 inquiry
corn and its processed products endogenous gene,pCaMV35S,pFMV35S,tNOS,NPTⅡ,BAR,PAT,GOX,CP4-EPSPS,,CTP2-CP4-EPSPS,Cry3A,tCaMV35S,PMI,CryIA(b),CRY1Ac,pRice-Eactin
rapeseed and its processed products endogenous gene,pCaMV35S,pFMV35S,tNOS,NPTⅡ,BAR,PAT,GOX,CP4-EPSPS,CTP2-CP4-EPSPS,pNOS,pSsuAra,pTA29,tCaMV35S,tE9,tOCS,tg7
rice and its processed products endogenous gene,pCaMV35S,tNOS,BAR,CryIA(b),CRY1Ac
cotton and its processed products endogenous gene,pCaMV35S,pFMV35S,tNOS,NPTⅡ,BAR,PAT,CP4-EPSPS,pUbi, tE9,CryIA(b),CRY1Ac
potatoes and their processed products endogenous gene,pCaMV35S,pFMV35S,pCaMV35S,pFMV35S,tNOS,NPTⅡ,CP4-EPSPS,Cry3A,pNOS
flax and its processed products endogenous gene,pCaMV35S,pFMV35S,tNOS,NPTⅡ
sugarbeet and its processed products endogenous gene,pCaMV35S,pFMV35S,tNOS,NPTⅡ,PAT,CP4-EPSPS,CTP2-CP4-EPSPS
alfalfa and its processed products endogenous gene,pFMV35S,CTP2-CP4-EPSPS,tE9
tomato pCaMV35S,pFMV35S,tNOS,NPTⅡ,CRY1Ac
apple pCaMV35S,pFMV35S,tNOS,NPTⅡ
chicory pCaMV35S,pFMV35S,tNOS,BAR,NPTⅡ
bentgrass pCaMV35S,pFMV35S,tNOS,CP4-EPSPS
tobacco pCaMV35S,pFMV35S,tNOS,NPTⅡ
plum pCaMV35S,pFMV35S,tNOS,NPTⅡ
muskmelon pCaMV35S,pFMV35S,tNOS,NPTⅡ
papaya pCaMV35S,pFMV35S,tNOS,NPTⅡ
wheat pCaMV35S,pFMV35S,tNOS,CP4-EPSPS
eggplant pCaMV35S,pFMV35S,tNOS,NPTⅡ,CRY1Ac
eucalyptus pCaMV35S,pFMV35S,tNOS,NPTⅡ

Identification of Transgenic Lines

If further strain identification is needed for the samples with positive detection of exogenous genes, or known positive samples, the specific fragments of the strains are amplified by line-specific real-time fluorescent PCR. According to the results of PCR amplification (real-time fluorescent amplification curve), the components of the samples are determined.

species selected genes price
soybean GTS40-3-2,MON89788,A2704-12,A5547-127,DP305423,DP356043,MON87701,CV127,MON87705,MON87769,FG72,MON87708 DAS-81419-2,DAS-68416-4,DAS-44406-6 inquiry
corn GA21,NK603,Bt11,Bt176,MON810,MON863 T25,CBH351,TC1507,59122,MIR604,3272 LY038,MON89034,MON88017,98140,MIR162 DAS-40278-9,MON87460,DAS59132,(Event 32) DAS-06275-8,TC-6275,5307,MON 87427
cotton MON531,MON1445,MON15985,MON88913 LLCotton25,GHB614,281-24-236,3006-210-23 T304-40,GBH119
rice TT51-1(Bt63),LLRice62,LLRice601,114-7-2 Kefeng 6,Kefeng 8,KMD1
rape RT73(GT73),MS8,MS1,RF1,RF2,RF3,Oxy235 T45(HCN28),Topas19/2 (HCN92),DP-073496-4 MON88302,DP-073496-4
potato EH92-527-1
flax Fp967
sugarbeet H7-1
 

Related services:

suspension culture of plant cells service

model crop genetically modified service

construction of plant overexpression vector and interference vector service