Zoonbio can transform Ben's tobacco and common tobacco by Agrobacterium tumefaciens mediated method. The fastest period is 2.5 months. Customers can provide plasmids, Agrobacterium and so on. We complete the whole experimental process for you from constructing vectors to obtaining transgenic seedlings.
| tobacco genetically modified services project | ||
| service items | service content | cycle |
| genetic transformation of tobacco | 1.vector construction 2.acquisition of tobacco aseptic vaccine 3.agrobacterium-mediated transformation and screening of tobacco 4.callus induction and adventitious bud differentiation of positive leaf disc 5.rooting and strong seedling of regenerated green seedlings 6.detection of exogenous genes in T0 generation transgenic seedlings by PCR |
3-4 months |
service description
transgenic experiment:
1.receptor Material Varieties: We provide our own varieties and other varieties as acceptor materials.
2.receptor material type: Tobacco leaf.
experiments quantitative explanation:
1.Vector construction: 1 vector was constructed for each foreign gene, one time.
2.Gene transformation: 1 kind of receptor material was transformed by each kind of transgene vector, one time in total.
provide results
1.Some pictures of genetic transformation and screening.
2.Agarose gel electrophoresis of PCR gene in T0 transgenic seedlings.
3.T1 Generation Transgenic Seeds: Five or more T1 Generation Seeds were obtained from each transformation experiment.
Zoonbio organism was transformed into Arabidopsis thaliana by Agrobacterium-mediated method. Customers can provide target genes, plasmids, Agrobacterium and so on. We complete the whole experimental process for you from constructing vectors to obtaining transgenic seedlings.
| arabidopsis transgenic service project | ||
| service items | service content | cycle |
| genetic transformation of arabidopsis thaliana | 1.vector construction 2.plant culture of arabidopsis thaliana 3.agrobacterium-mediated genetic transformation and screening 4.screening of T0 generation seeds 5.pure line screening and detection of exogenous Genes by PCR |
2-3 months |
service description
1.receptor material varieties: Columbia and other varieties are receptor materials.
2.description of experiments number:One receptor material was transformed into each transgenic vector once.
provide results
1.Some pictures of genetic transformation and screening.
2.Agarose gel electrophoresis of PCR gene in T0 transgenic seedlings.
3.T1 Generation Transgenic Seeds: Five or more T1 Generation Seeds were obtained from each transformation experiment.
Related services：
suspension culture of plant cells service
construction of plant overexpression vector and interference vector service
subcellular localization of plant proteins service