Suspension culture of biological cells refers to suspension culture of plant cells or small cell aggregates in liquid medium on shaking bed. Compared with traditional whole plant materials, plant cell suspension system has been widely used in the study of physiology, cytology, biochemistry, developmental biology, genetics and molecular biology because of its good dispersion, similar cell shape and cell mass size, rapid growth, good repeatability and easy control. It can not only be directly used for protoplast isolation, culture, hybridization, gene transfer, production of secondary metabolites, etc., but also for screening expected mutants at the cell level in a short time. Therefore, plant suspension cell culture has become one of the most useful means in plant biotechnology.
Our NBC Cell Line uses native tobacco as material, induces callus formation by aseptic culture, and suspension domestication culture for more than 20 generations. It has the advantages of rapid growth and stable system. Compared with traditional plant materials, its advantages of good dispersion and easy operation can significantly reduce the experimental errors caused by uneven sampling of plant materials and increase the experimental persuasion.
40-fold microscopic observation and shaking flask culture of NBC cells
|It can be applied to the study of plant cytology, metabolic pathway, the role of plant stress factors, gene editing, transient expression of exogenous genes, plant-microbial interaction and so on.|
images of subcellular localization of transient expression of GFP mediated by Agrobacterium tumefaciens
|1.fast growth rate|
|The doubled biomass of NBC suspension cultured cells proliferated for 7 days, which was obviously better than the growth rate of tissue culture seedlings and callus, and could greatly shorten the experimental period.|
|The dispersion and homogeneity of suspension cells are much better than those of traditional plant materials, so the experiment has good repeatability and more convincing results. In the experiment, because of the large specific surface area and small particle size, many experimental errors caused by material inhomogeneity are avoided. The NBC cells of Zoonbio were domesticated for more than 20 generations, and the cell state was more stable.|
|NBC suspension cells have strong tolerance, and can survive under temperature stress, hypoxia and lack of nutrients. The tolerance to many antimicrobial agents and experimental reagents is also good. The tolerance to microbial infection is higher than that of mammalian and insect cells, so it can be co-cultured with microorganisms for plant-microbial interaction experiments.|
|4.optimizable experimental process|
|As a cell-level experimental material, NBC has obvious advantages of fast growth, fast proliferation, strong cell viability and fast metabolism, which can greatly shorten the experimental cycle. Moreover, it has good dispersibility and saves a lot of steps of material treatment, which can simplify the experimental operation. As a cell-level research material, compared with protoplasts, it saves the steps of protoplast preparation, and the cell viability of suspension cells is stronger. It is easier to survive than protoplasts in experimental operation.|
|5.It can be used for plant regeneration|
|NBC suspension cells belong to embryogenic cells of native tobacco, which have high totipotency and can be used for plant regeneration. It can save the time of callus induction and shorten the experimental period obviously when used in transgenic experimental materials.|
ZN01 medium is a clear chemical composition medium developed by Zoonbio for NBC cells. It is stable in batches and suitable for the growth of NBC cells in our company.
The ZN01 medium has complete nutritional components and has been sterilized. It can be used directly.
In addition to providing NBC cell materials, Zoonbio can also develop special plant cell lines according to customer needs, and provide customized services for the overall experiment of plant-related research.
construction of plant overexpression vector and interference vector service
model crop genetically modified service
subcellular localization of plant proteins service