Characteristic Vector
1
vector characteristics:
1.Carries the cspA low temperature induction promoter, 11 degrees low temperature induction, promotes protein dissolution, increases the probability of soluble protein.
2.SD sequence is carried to increase the efficiency of protein codon translation.
3.TEE sequence was constructed to enhance transcription and protein expression.
2
vector characteristics:
1.The pelB signal peptide can secrete the expressed protein to the periplasm, which is conducive to the correct folding of the protein and the formation of disulfide bond, and greatly improve the probability of the expression of soluble active protein.
2.T7 promoter can activate transcription efficiently and increase protein expression level.
3
vector characteristics:
1.Carries the cspA low temperature induction promoter, 11 degrees low temperature induction, promotes protein dissolution, increases the probability of soluble protein.
2.The SUMO fusion protein is carried to form a three-level spatial structure, which is helpful for the correct folding of the protein.
3.SUMO specifically cleaved the fusion protein, and the N-terminal of the recombinant protein had no amino acid residues.
