pichia pastoris protein expression service丨Zoonbio

Summary

Yeast expression system is widely used ins industrial microorganisms. It has the advantages of short prokaryotic expression cycle, low cost and easy operation, and is easy to be enlarged and cultured at industrial level. At the same time, it has the ability of eukaryotic cells to fold and glycosylate proteins, which greatly improves the solubility of proteins and provides a powerful tool for studying the physiological activity of proteins.

Zoonbio has rich host cell resources and vector selection experience, and has developed innovative pYE-GAPalpha vector with GAP promoter. The procedure of protein induction was further improved, and the cell density and protein yield were effectively increased without changing the culture medium or detecting the methanol content in the process of protein induction.

Characteristic Carrier

1.GAP (3-phosphate glyceraldehyde dehydrogenase) promoter:methanol-free induction system.
2.It secretes its own signal peptide, which is beneficial to extracellular expression.
3.With its own His label protein, it is convenient for purification and detection.
    4.Zeocin (bleomycin) resistance can be used as both screening resistance and marker for high copy screening.
5.With PUC replicon, the plasmid can proliferate in high copy form in E. coli.

case

electrotransfer yeast and screening of positive strains

conventional positive clone plate	high copy positive clone plate	PCR detection of positive strains	detection of positive clonal expression product Dot-Blot

protein expression and purification

The molecular weight of the target protein is about 16KD, the expression yield is about 1.5mg/L, and the purity of the protein is about 80%.	The molecular weight of the target protein is about 35KD, the expression yield is about 2.1 mg/L, and the purity of the protein is about 90%.	The molecular weight of the target protein is about 92 KD, the expression yield is about 1.1 mg/L, and the purity of the protein is about 90%.