ELISA common method|zoonbio

The common methods of ELISA are detailed in the following table.

ELISA	Purpose	Required reagent	Evaluation
Indirect ELISA	antibody screening,epitope location	purified or semi purified antigens;test solution containing antibody;enzyme complexes linked to immunoglobulins of immune animals	no need to use pre-existing specific antibodies;requires a relatively large number of antigens
Direct competitive ELISA	antigen screening，detection of soluble antigens	purified or semi purified antigens;test solution containing antibody;enzyme antibody complex for antigen specificity	fast detection in 2 steps;excellent determination of antigen cross reactivity
Antibody Sandwich Method	antigen screening，detection of soluble antigens	capture antibody(purified or semi purified specific antibody);test solution containing antigen;enzyme antibody complex for antigen specificity	the most sensitive antigen detection;a relatively large number of purified or semi purified specific antibodies are required(capture antibody)
Double antibody sandwich method	antibody screening,epitope location	capture antibody;immunoglobulins, species-specific, immunoglobulin containing antigens;enzyme antibody complex for antigen specificity	antigens that do not need to be purified;the detection process is relatively long and needs 5 steps
Direct cell method	antigen expression of screening cells,detection of cellular antigen expression	cells expressing antigens of interest;enzyme antibody complex for cell antigen specificity	sensitive detection of mass screening;insensitive to the heterogeneity of mixed cell expression
Indirect cell method	screening antibodies against cell antigens	cells for immunity;test solution containing antibody;an enzyme complex that binds immunoglobulins of an immunized species	Specific antibodies may not be detected when cell surface antigen expression is at low density.