Ⅰ.Separation of gel solution by Tris- glycine SDS-PAGE polyacrylamide gel electrophoresis.

		The volume required for each component in different volume (ml) gels (ml)
	solution composition	5	10	15	20	25	30	40	50
6%
	water	2.6	5.3	7.9	10.6	13.2	15.9	21.2	26.5
	30% acrylamide solution	1	2	3	4	5	6	8	10
	1.5 mol/L Tris (pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
	10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	10% ammonium persulfate	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	TEMED	0.004	0.008	0.012	0.016	0.02	0.024	0.032	0.04
8%
	water	2.3	4.6	6.9	9.3	11.5	13.9	18.5	23.2
	30% acrylamide solution	1.3	2.7	4	5.3	6.7	8	10.7	13.3
	1.5 mol/L Tris (pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
	10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	10% ammonium persulfate	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	TEMED	0.003	0.006	0.009	0.012	0.015	0.018	0.024	0.03
10%
	water	1.9	4	5.9	7.9	9.9	11.9	15.9	19.8
	30% acrylamide solution	1.7	3.3	5	6.7	8.3	10	13.3	16.7
	1.5 mol/L Tris (pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
	10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	10% ammonium persulfate	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	TEMED	0.002	0.004	0.006	0.008	0.01	0.012	0.016	0.02
12%
	water	1.6	3.3	4.9	6.6	8.2	9.9	13.2	16.5
	30% acrylamide solution	2	4	6	8	10	12	16	20
	1.5 mol/L Tris (pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
	10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	10% ammonium persulfate	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	TEMED	0.002	0.004	0.006	0.008	0.01	0.012	0.016	0.02
15%
	water	1.1	2.3	3.4	4.6	5.7	6.9	9.2	11.5
	30% acrylamide solution	2.5	5	7.5	10	12.5	15	20	25
	1.5 mol/L Tris (pH8.8)	1.3	2.5	3.8	5	6.3	7.5	10	12.5
	10% SDS	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	10% ammonium persulfate	0.05	0.1	0.15	0.2	0.25	0.3	0.4	0.5
	TEMED	0.002	0.004	0.006	0.008	0.01	0.012	0.016	0.02

Ⅱ.Preparation of 6% Tris- glycine SDS-PAGE polyacrylamide gel electrophoresis solution for 5% laminated adhesive.

		The volume required for each component in different volume (ml) gels (ml)
	solution composition	1	2	3	4	5	6	8	10
	water	0.68	1.4	2.1	2.7	3.4	4.1	5.5	6.8
	30% acrylamide solution	0.17	0.33	0.5	0.67	0.83	1	1.3	1.7
	10% SDS	0.01	0.02	0.03	0.04	0.05	0.06	0.08	0.1
	TEMED	0.001	0.002	0.003	0.004	0.005	0.006	0.008	0.01