Common labels of recombinant protein expression

Protein tag refers to the protein or polypeptide fused with the target protein during the preparation of the recombinant protein. The functions of tag protein generally include: promoting the solubility and stability of the target protein; facilitating the detection of the target protein; facilitating the purification of the target protein. With the development of research and technology, various labels come into being, and various labels can meet different experimental purposes.

Introduction to common labels

Label	Sequence	Molecular Weight
6*His	HHHHHH	0.84kd
Flag	DYKDDDDK	1.01kd
GST	MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEF PNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGV SRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDAL DVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGG GDHPPKSDLVPRGSPEFPGRLERPHRD	26kd
FC	PRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVS EDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTD FMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSY SCSVVHEGLHNHHTTKSFSRTPGK	26kd
c-Myc	EQKLISEEDL	1.2kd
HA	YPYDVPDYA	11kd
Sumo	MNWSHPQFEKSSGSSGGHHHHHHGGSGGSGSDSEVNQEAKPEV KPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKE MDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGG	14kd
Arg6	RRRRR	0.86kd
TrxA	MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAA TKVGALSKGQLKEFLDANLA	14kd
CBP	KRRWKKNFIAVSAANRFKKISSSGAL	4kd
MBP	MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKL EEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQD KLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEI PALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGK YDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFN KGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGV LSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVAL KSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVI NAASGRQTVDEALKDAQT	40kd
Avi	GLNDIFEAQKIEWHE	18kd
eGFP	MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKS AMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFK EDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGS VQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITLGMDELYK.	27kd
eCFP	MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKS AMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFK EDGNILGHKLEYNYISHNVYITADKQKNGIKANFKIRHNIEDGSV QLADHYQQNTPIGDGPVLLPDN HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK	27kd