custom protein expression service-Zoonbio

Summary

• Over 10 years' experience recombinant proteins production.
• One-stop service from gene to protein.
• Multiple protein expression systems: bacterial, yeast, baculovirus-insect and mammalian expression system.
• Abundant R & D ability with over 1000 new proteins per year and quick troubleshooting.
• Close to 100 bioreactors with various volumes ranging from 2 to 1000 L to meet high-throughput and large-scale production.

Six Systems

1

Mammalian Transient Protein Expression Service

Transient transfection cells
High expression system of pMZ-X3
Selection of multisignal peptide

2

Baculovirus-Insect Cell Protein Expression Service

Sf9/high5 cell line
Highly secretory expression scheme
Bimolecular co-expression

3

E.coli Protein Expression Service

Prokaryotic expression of AE host
Low Temperature Induction System of 11 Degree C
Supernatant soluble expression scheme

4

L.Lactis Protein Expression System

Nisin-induced Lactobacillus protein expression
Food-level expression
Controllable endogenous protease

5

Stable Cell Line Development Service

GS/DHFR Pressure Screening Gene Expression System
10 Generation Stability Sequencing
Monoclonal Batch Culture and Mycoplasma Detection

6

Yeast Protein Expression Service

Methanol-free induction system
Screening of high-copy strains
Secretory expression scheme

Other

1

viral clearance validation service

Zoonbio has a second-level biological laboratory in Beijing, which is designed and managed strictly in accordance with GLP specifications.

2

endotoxin removal related

Summary of Endotoxin Removal Methods and Notices for Endotoxin Removal.

3

cell bank testing service

short detection period
method maturity
rich experience

Protein Requirements

Tag	Amount	Purity Options	Endotoxin Level Options
Small Tag(e.g. His, Flag) Large Tag(e.g. GST, Fc)	• Bacterial Expression for 1~10mg • Yeast Expression for 1~5mg • Insect and Mammalian system expression for 50ug~500ug • Large amount can be customized after expression test.	80% 85% 90% 95%	≤1.0 EU/μg ≤0.1 EU/μg

Service Process

1.Pre-project analyze protein sequence.

2.• Plasmid Prep • Gene synthesis or Cloning • Customer supply

3.Protein Expression Testing

4.• Protein Purification • Tag Removal (optional) QC & Delivery

What will you get from us?

• Expression Vector, Gene synthesis or Cloning by us.

• Purified protein, protein dissolved in buffer solution or in Freeze-dried powder.

• Certificate of Analysis, contains all test data for your customized vector and protein.

Service features

• We developed a low temperature-inducible expression plasmid pCznI by our self, which greatly enhances the solubility of proteins expressed by E. coli.

• We have successfully expressed membrane proteins, viral capsid proteins and proteins with a protein content of up to 250 kd.

• We have 20-1000L fermenters that can produce proteins on a large scale.

Service Details

Expression system	Plasmid Prep	Protein Expression Testing	Timeline	Deliverables
Bacterial Protein Expression	• Customer supply(Please provide detail information of your plasmid) • Gene synthesis and Codon optimization(optional) • Sub cloning into appropriate expression vector • Plasmid Prep and QC	• Plasmid transformed Escherichia coli • Protein expression evaluation and optimization • Protein expression using optimized conditions • Protein Purification, tag removal (optional) • QC & Delivery	2weeks (Does not contain Plasmid Prep)	• Purified Protein in buffered solution with your specified purity / in Freeze-dried powder • Expression Vector and optimized gene sequence report and QC data • COA for purified protein
Yeast Protein Expression		• Plasmid electrotransformating Pichia Pastoris • Positive clone strain screening • High copy strain screening (optional) • Yeast induced expression optimization • Protein expression using optimized conditions • Protein Purification • QC & Delivery	4-5weeks (Does not contain Plasmid Prep)
Insect Protein Expression		• Recombinant Bacmid DNA Generating • Recombinant Bacmid transfecting Insect Cells, expression conditions optimization • P2 generation of Recombinant Baculovirus transfecting Insect Cells to express protein in optimized conditions • Protein Purification • QC & Delivery	4weeks (Does not contain Plasmid Prep)
Mammalian Protein ExpressionProtein		• High purity endotoxin free plasmid extraction • Plasmid transfection of mammalian cells • Protein expression evaluation and optimization • Protein expression using optimized conditions • Protein Purification • QC & Delivery	4weeks (Does not contain Plasmid Prep)