• Over 10 years' experience recombinant proteins production.
• One-stop service from gene to protein.
• Multiple protein expression systems: bacterial, yeast, baculovirus-insect and mammalian expression system.
• Abundant R & D ability with over 1000 new proteins per year and quick troubleshooting.
• Close to 100 bioreactors with various volumes ranging from 2 to 1000 L to meet high-throughput and large-scale production.

Six Systems

mammalian transient protein


Mammalian Transient Protein Expression Service

Transient transfection cells
High expression system of pMZ-X3
Selection of multisignal peptide

Read More
baculovirus-Insect Cell Protein


Baculovirus-Insect Cell Protein Expression Service

Sf9/high5 cell line
Highly secretory expression scheme
Bimolecular co-expression

Read More
E.coli protein


E.coli Protein Expression Service

Prokaryotic expression of AE host
Low Temperature Induction System of 11 Degree C
Supernatant soluble expression scheme

Read More

L.Lactis protein


L.Lactis Protein Expression System

Nisin-induced Lactobacillus protein expression
Food-level expression
Controllable endogenous protease

Read More
stable cell line


Stable Cell Line Development Service

GS/DHFR Pressure Screening Gene Expression System
10 Generation Stability Sequencing
Monoclonal Batch Culture and Mycoplasma Detection

Read More
yeast protein


Yeast Protein Expression Service

Methanol-free induction system
Screening of high-copy strains
Secretory expression scheme

Read More


virus inactivation


viral clearance validation service

Zoonbio has a second-level biological laboratory in Beijing, which is designed and managed strictly in accordance with GLP specifications.

Read More
endotoxin removal related


endotoxin removal related

Summary of Endotoxin Removal Methods and Notices for Endotoxin Removal.

Read More
endotoxin removal related


cell bank testing service

short detection period
method maturity
rich experience

Read More

Protein Requirements

Tag Amount Purity Options Endotoxin Level Options
Small Tag(e.g. His, Flag)
Large Tag(e.g. GST, Fc)
• Bacterial Expression for 1~10mg
• Yeast Expression for 1~5mg
• Insect and Mammalian system
expression for 50ug~500ug
Large amount can be customized after expression test.
≤1.0 EU/μg
≤0.1 EU/μg

Service Process

1.Pre-project analyze protein sequence.

2.• Plasmid Prep  • Gene synthesis or Cloning  • Customer supply

3.Protein Expression Testing

4.• Protein Purification  • Tag Removal (optional)  QC & Delivery

What will you get from us?

• Expression Vector, Gene synthesis or Cloning by us.

• Purified protein, protein dissolved in buffer solution or in Freeze-dried powder.

• Certificate of Analysis, contains all test data for your customized vector and protein.

Service features

• We developed a low temperature-inducible expression plasmid pCznI by our self, which greatly enhances the solubility of proteins expressed by E. coli.

• We have successfully expressed membrane proteins, viral capsid proteins and proteins with a protein content of up to 250 kd.

• We have 20-1000L fermenters that can produce proteins on a large scale.

Service Details

Expression system Plasmid Prep Protein Expression Testing Timeline Deliverables
Bacterial Protein Expression • Customer supply(Please provide detail information of your plasmid)
• Gene synthesis and Codon optimization(optional)
• Sub cloning into appropriate expression vector
• Plasmid Prep and QC
• Plasmid transformed Escherichia coli
• Protein expression evaluation and optimization
• Protein expression using optimized conditions
• Protein Purification, tag removal (optional)
• QC & Delivery
2weeks (Does not contain Plasmid Prep) • Purified Protein in buffered solution with your specified purity / in Freeze-dried powder
• Expression Vector and optimized gene sequence report and QC data
• COA for purified protein
Yeast Protein Expression • Plasmid electrotransformating Pichia Pastoris
• Positive clone strain screening
• High copy strain screening (optional)
• Yeast induced expression optimization
• Protein expression using optimized conditions
• Protein Purification
• QC & Delivery
4-5weeks (Does not contain Plasmid Prep)
Insect Protein Expression • Recombinant Bacmid DNA Generating
• Recombinant Bacmid transfecting Insect Cells, expression conditions optimization
• P2 generation of Recombinant Baculovirus transfecting Insect Cells to express protein in optimized conditions
• Protein Purification
• QC & Delivery
4weeks (Does not contain Plasmid Prep)
Mammalian Protein ExpressionProtein • High purity endotoxin free plasmid extraction
• Plasmid transfection of mammalian cells
• Protein expression evaluation and optimization
• Protein expression using optimized conditions
• Protein Purification
• QC & Delivery
4weeks (Does not contain Plasmid Prep)