Molecular biological techniques can help us to detect DNA and RNA in samples qualitatively and quantitatively.
For example, fluorescent quantitative PCR can detect the content change of a gene in different samples, Southern blot can detect the copy number of a target gene in genomic DNA of samples, and 16S, 18S gene sequence information can be used to judge the pending detection.
Species and genus of fungi.
Molecular biological techniques were used to analyze the content, size, abundance and function of the genes in the detected samples at the molecular level, such as southern blot, northern blot, qPCR, EMSA. DNA-pull-down, GWAS, etc., the company can provide professional technical services.
Use of digoxigenin labelled probes,sshort cycle and high cost performance ratio.
From genomic DNA extraction, probe synthesis to hybridization experiment, a perfect hybridization picture can be delivered in 15 working days.
Use of digoxigenin labelled probes,sshort cycle and high cost performance ratio.
In 15 working days, RNA extraction, probe design, synthesis and labeling, and Northern Blot detection can be completed.
We can use sybrgreen method or taqman method to quantitatively determine or type DNA/RNA by real-time-PCR, and design characteristic internal parameters according to sample types.
We can use sybrgreen method or taqman method to quantitatively determine or type DNA/RNA by real-time-PCR, and design characteristic internal parameters according to sample types.
Zoonbio has rich experience in the field of molecular biology detection, and can provide customers with affordable, quality assurance fluorescence in situ hybridization (FISH) technology services.
Soil samples, intestinal samples, fecal samples, sewage treatment materials, food, swamp, water samples before and after different treatments, air, koji and so on can be detected.
Zoonbio can provide high-quality, fast and professional luciferase reporter gene detection services. We have worked with customers for many times to complete all the work from experimental design to paper publication, and have rich practical experience.
Zoonbio uses high-throughput fluorescence quantitative PCR technology to detect the expression profile of resistance genes in the environment and analyze the level of resistance genes in the environment.
Zoonbio primers optimized by a large number of experiments are suitable for more than 99% of microorganisms, and the identification success rate is more than 95%.
Some SNPs affect gene function directly and lead to changes of biological character. SNPs are abundant in genome and widely used in study of population genetics and disease genes.
The resequencing project builds a database and sequences the genomic DNA of the target sample, and then compares the sequencing data to the reference genome through bioinformatics analysis, so as to obtain a large amount of genetic variation information.
The expression of lentivirus is stable and high. Zoonbio uses lentivirus system to produce replication defective lentivirus particles with high titer, which can effectively transduce almost all types of divided and non divided cells.
Telomere DNA is composed of a large number of simple tandem repeats, which are synthesized by telomerase. In the process of plant growth and development, there will be changes in length, repeat sequence and rearrangement, which are important indicators of different physiological stages of plants.
Zoonbio adopts 293A adenovirus packing cell line,used to produce replication deficient (E1 and E3 deletion) adenoviruses with high titer for expression of foreign genes.